Abzyme offers endotoxin-free protein production and purification services for single domain antibodies, scFv and recombinant IgG antibodies. CHO and HEK cell lines are principally used for IgG production, and all VH, VHH and ScFvs are generated in E. coli. Mammalian cells are recommended when glycosylated antibodies are desired. Production scales from 250 mL to 100 L are readily available.
All antibodies are purified by affinity chromatography and characterized by SDS-PAGE, ELISA, and/or Western blot. Additional SPR-based characterization to determine ka, kd, and KD will be performed upon request.